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If you notice that the green volume bar isn't moving when you are speaking, this indicates that the microphone isn't recording your audio.
A good way to test if the microphone is working is to go to this page:
If it is working you will see movement when you speak, if there is no movement on the screen, then your microphone isn't working in Google Chrome.
The first thing to try is to restart your computer. This will usually get around any issues you were having, but if that fails to get things working there are a number of other things you can try...
Here is a list of known apps/extensions that can cause issues with audio recording in Chrome: